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Complementary DNA (cDNA)

Complementary DNA (cDNA) is DNA synthesized from a mature mRNA template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase. cDNA is often used to clone eukaryotic genes in prokaryotes. When scientists want to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell. cDNA is also produced by retroviruses (such as HIV-1, HIV-2, Simian Immunodeficiency Virus, etc.) which is integrated into its host to create a provirus.

Andrea Charles

Professor René Bernards, Head of the Division/Group Leader, Molecular Carcinogenesis at The Netherlands Cancer Institute, joins Andrea Charles from Pharma IQ, to discuss current trends in next-generation DNA sequencing in oncology research,...

Contributor: Andrea Charles
Tue, 09/13/2011
Pharma IQ

Professor Ann Logan, Lead Professor, University of Birmingham, joins Helen Winsor Pharma IQ, to discuss next-generation DNA sequencing. Logan reports on her laboratory’s primary research into the role of the intravitreal inflammation in the axon...

Contributor: Pharma IQ
Sun, 12/12/2010
Pharma IQ

Although more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA abundance information all provide valuable insights...

Contributor: Pharma IQ
Sun, 08/08/2010