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iam eswa from oman we analyse cefixime product by hplc used short column (100*4.6*5) in that resolution is NLT 2.after 200 injections we cannot get it ,then theritical plate was also gradually decrease,in the mobile phase tetrabutylammnium hydroxide(40%aqueous) after 200 injection required new column,how can overcome this problem

Author: eswarforyou
Posted: 12/04/2015