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Spectrophotometry is defined as a process in which light of known intensity and wavelength is passed through a solution and used to estimate the levels of substances dissolved in that solution. Different materials absorb light of specific wavelength and the more of that substance present, the more light of that wavelength will be absorbed. If we measure the amount of light of a particular wavelength which passes through the substance, we can calculate the amount absorbed and therefore estimate the levels of the material absorbing the light.