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DNA Sequencing

DNA Sequencing refers to sequencing methods for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA. Two common methods available are The Maxam Gilbert technique, which uses chemicals to cleave DNA into fragments at specific bases; or, most commonly, the Sanger technique (also called the di-deoxy or chain-terminating method) which uses DNA polymerase to make new DNA chains, in the presence of di-deoxynucleotides (chain terminators) to stop the chain randomly as it grows. In both cases, the DNA fragments are separated according to length by polyacrylamide gel electrophoresis, enabling the sequence to be read directly from the gel.

An Interview with Gerd Maass, VP for R&D at Roche Pharmaceuticals: Chapter 1 - DNA Sequencing

Gerd Maass, Vice President for R&D at Roche Pharmaceuticals, shares his views on advances in companion diagnostics, DNA sequencing and biobanking. This segment on DNA sequencing addresses claims that use of DNA sequencing systems in labs has...

04/12/2011 08:00:00 PM EDT
Accelerating Biological and Biomedical Research

The outlook for genome research looks bright. A technological evolution in the past few years has reduced the cost of DNA sequencing, accelerating the possibilities for future research. The National Human Genome Research Institute (NHGRI) in the...

Contributor: Pharma IQ
11/28/2010 07:00:00 PM EST
Pharma IQ

Although more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA abundance information all provide valuable insights...

Contributor: Pharma IQ
08/09/2010 08:00:00 PM EDT